M. Manuela R. da Fonseca

(Associate professor)

Status at the IBB – Institute for Biotechnology and Bioengineering:

·         Senior researcher

Status at Centre for Biological and Chemical Engineering:

·           Senior researcher    

Education:

·         Ph. D. in Chemical Engineering, UMIST, U.K. , 1983   

·         M.Sc. in Advanced Chemical Engineering, UMIST, U.K. , 1978

·         Graduation in Industrial Chemical Engineering, IST, 1974

Positions and activities:

·         Associate Professor, IST, 1998 -

·        Assistant Professor, IST, 1986 – 1998

·         Assistant Professor, School of Pharmacy , University of Lisbon , 1984 - 1986  

·         Teaching Assistant, School of Pharmacy , University of Lisbon , 1975 - 1977, 1982 - 1983

Area of scientific activity:

·         Biological Engineering

Domain of specialization:

·         Biological Reactors

Interests:

·         Mass Transfer in multiphasic bioreactors

·         Cell membrane composition as modulated by the environment

·         Biotransformations in aqueous / organic media with whole cells; bioreactors; product recovery

·         Production of structured triglycerides with immobilised lipases

·         Production of polyhydroxyalkanoates from industrial waste

Scientific Societies Membership:

·         Sociedade Portuguesa de Biotecnologia  

·         European Federation of Biotechnology

Recent publications:

 P. Pires-Cabral, M. M. R. da Fonseca, S. Ferreira-Dias (2010) Esterification activity and operational stability of Candida rugosa lipase immobilized in polyurethane foams in the production of ethyl butyrate, Biochem. Eng. J., doi.org/10.1016/j.bej.2009.10.021

 P. Pires-Cabral, M. M. R. da Fonseca, S. Ferreira-Dias (2009) Synthesis of Ethyl butyrate in organic media catalyzed by Candida rugosa lipase immobilized in polyurethane foams: A kinetic study, Biochem. Eng. J. 43, 327-332.

J. M. B. T. Cavalheiro, M. C. M. D. de Almeida, C. Grandfils and M. M. R. da Fonseca (2009) Poly(3-Hydroxybutyrate) Production by Cupriavidus necator Using Waste Glycerol, Process Biochem. 44, 509-515.

N.M. Osório, E. Dubreucq, M.M.R.da Fonseca, S. Ferreira-Dias (2009 Operational stability of immobilised lipase/acyltransferase during interesterification of fat blends, Eur. J. Lipid Sci. Technol. 111, 358-367.

N.M. Osório, E. Dubreucq, M.M.R.da Fonseca, S. Ferreira-Dias (2009), Lipase/acyltransferase-catalysed interesterification of fat blends containing omega-3 polyunsaturated fatty acids, Eur. J. Lipid Sci. Technol. 111, 120-134.

A. S. Pires, A. C. Nascimento, F. van Keulen, M.M.R. da Fonseca, S. Ferreira-Dias (2008), Pattern-Recognition of Lipase-Catalysed or Chemically-Interesterified Fat Blends containing omega-3 Polyunsaturated Fatty Acids, Eur. J. Lipid Sci. Technol. 110, 893-904.

N. M. Osório, M.H. Ribeiro, M.M.R. da Fonseca, S. Ferreira-Dias (2008), Interesterification of Fat Blends rich in Omega-3 Polyunsaturated Fatty Acids Catalysed by Immobilized Thermomyces lanuginosa Lipase under High Pressure, J Mol Cat B: Enzymatic 52-53, 58-66.

P. Pires-Cabral, M.M.R. da Fonseca, S. Ferreira-Dias (2007) Modelling the Production of Ethyl Butyrate Catalysed by Candida rugosa Lipase Immobilised in Polyurethane Foams, Biochem. Eng. J. 33, 148-158.

Carla C. C. R. de Carvalho, Vanessa Fatal, Sebastião S. Alves and M. Manuela R. da Fonseca (2007) Adaptation of Rhodococcus erythropolis cells to high concentrations of toluene, Applied Microbiology and Biotechnology, 76, 1423-1430

 Carla C.C.R. de Carvalho, Marco P. C. Marques, Pedro Fernandes, M. Manuela R. da Fonseca (2007) Degradation of hydrocarbons and alcohols by Rhodococcus erythropolis DCL14: a comparison in scale performance, Biocatalysis and Biotransformation, 25(2-4), 144-150

Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2007) Preventing biofilm formation: promoting cell separation with terpenes, FEMS Microbiology Ecology, 61, 406-413

Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2007) Bacterial whole cell biotransformations: in vivo reactions under in vitro conditions, Dynamic Biochemistry, Process Biotechnology and Molecular Biology, 1(1), 32-39

Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2007) Assessment of three-dimensional biofilm structure using an optical microscope, BioTechniques, 42(5), 616-620

C.C.C.R. de Carvalho and M.M.R. da Fonseca, 2006, “Biotransformation of terpenes” (Review), Biotechnol. Adv. 24, 134-142.

N. M. Osório, M.M.R. da Fonseca, S. Ferreira-Dias, 2006, "Operational Stability of Thermomyces lanuginosa Lipase during Fats Interesterification in Continuous-Packed-Bed Reactors", Eur. J. Lipid Sc. Techn., 108, 545-553.

C.C.C.R. de Carvalho, B. Parreño-Marchante, G. Neumann, M.M.R. da Fonseca, H. J. Heipieper, 2005, “Adaptation of Rhodococcus erythropolis DCL14 to growth on n-alkanes, alcohols and terpenes”, Appl Microbiol Biotechnol 67, 383-388.

C.C.C.R. de Carvalho and M.M.R. da Fonseca, 2005, “Degradation of hydrocarbons and alcohols at different temperatures and salinities by Rhodococcus erythropolis DCL14", FEMS Microbiol Ecology 51, 389-399.

C.C.C.R. de Carvalho, M.P.C. Marques, P. Fernandes, M.M.R. da Fonseca, 2005, “A simple imaging method for biomass determination”, J Microbiol Meth 60, 135-140.

J.C. Rodrigues, A.C. Nascimento, A. Alves; N.M. Osório; A.S. Pires; J.H.Gusmão; M.M.R. da Fonseca, S. Ferreira-Dias, 2005, "Calibration of Near Infrared Spectroscopy for Solid Fat Content of Fat Blends Analysis using Nuclear Magnetic Resonance Data", Analytica Chimica Acta 544, 213-218.

N. M. Osório, J. H. Gusmão, M. M. R. da Fonseca, S. Ferreira-Dias, 2005, “Lipase-catalysed Interesterification of Palm Stearin with Soybean Oil in a Continuous Fluidised-bed Reactor”, Eur. J. Lipid Sc. Techn. 107, 455-463.

C.C.C.R. de Carvalho and M.M.R. da Fonseca (Review), 2005, “The remarkable Rhodococcus erythropolis”, Appl Microbiol. Biotechnol 67, 715-726.

P. Pires-Cabral, E. Dubreucq, M.M.R. da Fonseca, S. Ferreira-Dias, 2005, “Partitioning of Water in Organic Systems with Lipase Immobilised in Polyurethane Foams”, Biochem Eng J 26, 29-37.

C.C.C.R. de Carvalho, A. Cruz, B. Angelova, P. Fernandes, M.N. Pons, H.M. Pinheiro, J.M.S. Cabral and M.M.R. da Fonseca, 2004, “Behaviour of Mycobacterium sp. NRRL B-3805 whole cells in aqueous, organic-aqueous and organic media studied by fluorescence microscopy”, Appl. Microbiol Biotechnol 64, 695 - 701.

C.R.C. Calado, B.S.Ferreira, M.M.R. da Fonseca, J.M.S. Cabral, L.P.Fonseca, 2004, “Integration of the Production and the Purification Processes of Cutinase Secreted by a Recombinant Saccharomyces cerevisiae SU50 strain”, J. Biotechn. 109, 147-158.

B.S. Ferreira, C.R.C. Calado, F. van Keulen, L.P. Fonseca, J.M.S. Cabral and M.M.R. da Fonseca, 2004, “Recombinant Saccharomyces cerevisiae Strain Triggers Acetate Production to Fuel Biosynthetic Pathways”, J. Biotechn.109, 159-167.

L.S. Pinto, L.M. Vieira, M.N. Pons, M.M.R. Fonseca and J.C. Menezes, 2004, “Morphology and Viability Analysis of Streptomyces clavuligerus in Industrial Cultivation Systems”, Bioprocess Biosyst Eng. 26, 177-184.

C.C.C.R. de Carvalho and M.M.R. da Fonseca, 2004, “Principal Component Analysis Applied to Cell Behaviour in the Presence of Organic Solvents“,Biocat Biotrans 22, 203-214.

D.M.R. Mateus, S.S. Alves, M.M.R. da Fonseca, 2004, “Kinetics of L-tryptophan Production from Indole and L-Serine, Catalized by Whole Cells with Tryptophanase Activity”, J. Biosci. Bioeng 97, 289-293.

C.C.C.R. de Carvalho, A.A.R.L. da Cruz, M.N. Pons, H.M.R.V. Pinheiro, J.M.S. Cabral, M.M.R. da Fonseca, B.S.Ferreira, P.C.B. Fernandes, 2004,"Mycobacterium sp., Rhodococcus erythropolis and Pseudomonas putida behaviour in the presence of organic solvents", Microsc. Res.  Tech. 64, 215-222.

A. C. Nascimento, C.S. R. Tecelão, J. H. Gusmão , M. M. R. da Fonseca, S. Ferreira-Dias, 2004, “Modelling lipase-catalysed transesterification of fats containing omega-3 fatty acids monitored by their Solid Fat Content”, Eur. J. Lipid Sc. Techn. 106, 599-612.

C.C.C.R. de Carvalho and M.M.R. da Fonseca, 2004, “Solvent Toxicity in Organic-Aqueous Systems Analysed by Multivariate Analysis”, Bioprocess Biosyst Eng. 26, 361-375.

C.C.C.R. de Carvalho, M.M.R. da Fonseca, 2003, “A simple method to observe organic solvent drops with a standard optical microscope”, Microsc. Res.  Tech., 60,  465-466.

S. Ferreira-Dias, A.C. Correia, M.M.R. da Fonseca, 2003, “Response Surface Modelling of Glycerolysis Catalysed by Candida rugosa Lipase Immobilized in Different Polyurethane Foams for the Production of Partial Glycerides”, J. Mol. Catalysis B: Enzymatic 21, 71-80.

B.S. Ferreira, C.R.C. Calado, F. van Keulen, L.P. Fonseca, J.M.S. Cabral, M.M.R. da Fonseca, 2003, “Towards a Cost Effective Strategy for Cutinase Production by a Recombinant Saccharomyces cerevisiae: Strain Physiology Aspects”, Appl Microbiol Biotechnol, 61, 69-76.

C.C.C.R. de Carvalho and M.M.R. da Fonseca, 2003, “Principal Components Analysis as a Tool to Summarise Biotransformation Data: Influence on Cells of Solvent Type and Phase Ratio”, Biocat Biotrans.21, 305-314.

C.C.C.R. de Carvalho and M.M.R. da Fonseca, 2003, “Towards the bio-production of trans-carveol and carvone from limonene: induction after cell growth on limonene and toluene”, Tetrahedron: Asymmetry 14, 3925-3931.

B.S. Ferreira, F. van Keulen and M.M.R. da Fonseca, 2002,"A Microporous Membrane Interface for the Monitoring of Dissolved Gaseous and Volatile Compounds by on-line Mass Spectrometry", J. Membrane Science, 208, 49-56.

C.C.C.R. de Carvalho, M.M.R. da Fonseca, 2002, “Modelling the Bio-kinetic Resolution of Diastereomers Present in Unequal Initial Amounts”, Tetrahedron: Asymmetry, 13, 1637-1643.

C.C.C.R. de Carvalho, M.M.R. da Fonseca, 2002, “Maintenance of Cell Viability in the Biotransformation of (-)-Carveol with Whole Cells of Rhodococcus erythropolis”, J. Mol. Catalysis B: Enzymatic, 19-20, 389-398.

C.C.C.R. de Carvalho, M.M.R. da Fonseca, 2002 “Influence of Reactor Configuration on the Production of Carvone from Carveol by Whole Cells of Rhodococcus erythropolis DCL14”, J. Mol. Catalysis B: Enzymatic, 19-20, 377-387.

S. Ferreira-Dias, A.C. Correia, F.O. Baptista and M.M.R. da Fonseca, 2001,"Contribution of Response Surface Design to the Development of Glycerolysis Systems Catalyzed by Commercial Immobilized Lipases", J Mol Catalysis B: Enzymatic, 11, 699-711.

C.S.R. Tecelão, F. van Keulen and M.M.R. da Fonseca, 2001,"Development of a Reaction System for Selective Conversion of (-)-trans-carveol to (-)-Carvone with Whole Cells of Rhodococcus erythropolis DCL14", J Mol Catalysis B: Enzymatic, 11, 719-724.

N.M. Osório, S. Ferreira-Dias, J.H. Gusmão and M.M.R. da Fonseca, 2001,"Response Surface Modelling of the Production of w-3 Polyunsaturated Fatty Acids Enriched Fats by a Commercial Immobilized Lipase", J  Mol Catalysis B: Enzymatic, 11, 677-686.

B.S. Ferreira, F. van Keulen and M.M.R. da Fonseca, 2000, "On-line Monitoring of Dissolved Gases Using Microporous Membrane Inlet and Mass Spectrometry", Food Technol Biotechn, 38, 63-67.

C.C.R. de Carvalho, F. van Keulen, M.M.R. da Fonseca, 2000, "Production and Recovery of Limonene-1,2-diol and Simultaneous Resolution of a Diastereomeric Mixture of Limonene-1,2-epoxide with Whole Cells of Rhodococcus erythropolis DCL14", Biocat  Biotransf, 18, 223-235.

C.C.R. de Carvalho, F. van Keulen and M.M.R. da Fonseca, 2000,"Biotransformation of Limonene-1,2-epoxide to Limonene-1,2-diol by Rhodococcus erythropolis Cells. An Introductory Approach to Selective Hydrolysis and Product Separation", Food Technol Biotechn, 38, 181-185.