Carla
C.C.R. de Carvalho
(
Research Scientist)
Status at Centre for Biological and Chemical Engineering:
· Research
Scientist of
Environmental
and Ecoprocess Engineering Research Group
Education:
· Ph. D. in Biotechnology,
IST, 2003
·
M. Sc. in Biotechnology
(Biochemical Engineering), IST, 1999
·
Graduation
in Chemical Engineering, IST, 1998
Activities:
·(since March 2008) Research scientist (Environmental and Ecoprocess Engineering Research Group)
·(January 2007-February 2008) Post-doctoral project entitled “Study and modelling of gram-positive bacterial cellular membranes in natural and produced biofilms; application in biomedical, biotransformation and bioremediation systems” , under the supervision of Prof. M. Manuela R. da Fonseca and Dr. Herman J. Heipieper. IST, UTL, Lisbon , Portugal , and UFZ Centre for Environmental Research, Leipzig , Deutschland.
· (January
2003 - December 2006) Post-doctoral project entitled “Methods of
evaluation of the extent, thickness and physical structure of biofilms;
strategies for prevention and destruction in tubes of ultra-pure water
systems”, under supervision of Prof. M. Manuela R. da Fonseca
. . IST,
UTL,
· (January
2000 – December 2003) Ph.D. project entitled "Bioconversion of
(‑)-carveol to (-)-carvone catalysed by whole cells of Rhodococcus
erythropolis. Influence of reaction conditions on cell morphology and
physiology", under supervision of Prof. M. Manuela R. da Fonseca
. .
IST, UTL,
· (April 1997 – July 1997)
Study
entitled “Synthesis of a dipeptide (kyotorphin) in supercritical carbon
dioxide” (SOCRATES/ERASMUS program), under supervision of Prof. José
Luis Iborra.
Area
of scientific activity:
· Microreactors
· Biofilms
· Fluorescence microscopy and image analysis
· Biocatalysis, biotransformation and bioremediation
Domain of specialization:
· Biotechnology and Biochemical Engineering
Interests:
· Prevention
and Identification of Biofilms – Development of fast and reliable methods to
detect biofilms; prevention of cell adhesion to other cells and surfaces
· Fluorescence
Microscopy and Image Analysis – Monitoring cell viability, morphology and
energetic state; development of image analysis programs to quantify populational
and individual characteristics
· Biotransformation
– Production of high-value compounds, namely terpenes and terpenoids, from
low-value substrates in organic:aqueous systems using whole cells
· Design
of bioreactors, Bioreactor and Bioprocess Engineering – Development of
bioreactors and conditions that allow maintenance of high cell viability and
productivity
· Effect
of toxic compounds on bacterial cells – Impact of toxic compounds on cell
viability, morphology and metabolism
· Study
of the composition of bacterial membranes – Effect of stressful compounds and
conditions on cellular membranes
Participation
in Research Projects:
Investigation
grant in the european project “Biotransformation of Terpenes”
(BIO-CT95-0049) between February 1998 and June 1999.
Research
fellow in the project POCI/SAU-MMO/59370/2004 (Health Sciences – Molecular
Medicine), entitled “Efflux-pumps in drug resistance of Mycobacterium
tuberculosis: Molecular characterisation of the efflux mechanism and use of
efflux inhibitors as new anti-tubercular compounds”, since January 2006.
· Member
of the “Biochemical Engineering Section” of the European Federation of
Biotechnology
Recent publications:
Carla C. C. R. de Carvalho, Maria José Caramujo (2008) Ancient procedures for the high-tech world: health benefits and antimicrobial compounds from the Mediterranean empires, The Open Biotechnology Journal, 2, 235-246
Carla C. C. R. de Carvalho, Vanessa Fatal, Sebastião S. Alves and M. Manuela R. da Fonseca (2007) Adaptation of Rhodococcus erythropolis cells to high concentrations of toluene, Applied Microbiology and Biotechnology, 76, 1423-1430
Carla C.C.R. de Carvalho, Marco P. C. Marques, Pedro Fernandes, M. Manuela R. da Fonseca (2007) Degradation of hydrocarbons and alcohols by Rhodococcus erythropolis DCL14: a comparison in scale performance, Biocatalysis and Biotransformation, 25(2-4), 144-150
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2007) Preventing biofilm formation: promoting cell separation with terpenes, FEMS Microbiology Ecology, 61, 406-413
M.P.C. Marques, C.C.C.R. de Carvalho, M. J. C. Claudino, J.M.S. Cabral, P. Fernandes (2007) On the feasibility of the microscale approach for sterol bioconversion, Journal of Chemical Technology and Biotechnology, 82(9), 856-863
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2007) Bacterial whole cell biotransformations: in vivo reactions under in vitro conditions, Dynamic Biochemistry, Process Biotechnology and Molecular Biology, 1(1), 32-39
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2007) Assessment of three-dimensional biofilm structure using an optical microscope, BioTechniques, 42(5), 616-620
Carla C.C.R. de Carvalho (2007) Biofilms: recent developments on an old battle, Recent Patents on Biotechnology, 1(1), 49-57
Carla
C. C. R. de Carvalho and M. Manuela R. da Fonseca (2006) Biotransformation
of terpenes (Review), Biotechnology Advances, 24, 134-142.
Carla
C. C. R. de Carvalho and M. Manuela R. da Fonseca (2006) Carvone: why and
how should one bother to produce this terpene (Review) Food Chemistry,
95, 413-422.
Carla C. C. R. de Carvalho, Alessandro Poretti, M. Manuela R. da Fonseca
(2005) Cell adaptation to substrate, solvent and product: a successful
strategy to overcome product inhibition in a bioconversion system,
Applied Microbiology and Biotechnology, 69(3), 268-275.
Carla
C. C. R. de Carvalho and M. Manuela R. da Fonseca (2005) The remarkable Rhodococcus
erythropolis (Review) Applied Microbiology and Biotechnology, 67
(6), 715-726.
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca (2005) Degradation
of hydrocarbons and alcohols at different temperatures and salinities by Rhodococcus
erythropolis DCL14, FEMS Microbiology Ecology, 51, 389-399.
Carla C. C. R. de Carvalho, Marco P. C. Marques, Pedro Fernandes, M.
Manuela R. da Fonseca (2005) A simple imaging method for biomass
determination. Journal of Microbiological Methods, 60(1), 135-140.
Carla C. C. R. de Carvalho, Beatriz Parreño-Marchante, Grit Neumann, M.
Manuela R. da Fonseca, Hermann J. Heipieper (2005) Adaptation of Rhodococcus erythropolis DCL14 to growth on n-alkanes, alcohols and
terpenes. Applied Microbiology and Biotechnology, 67 (3), 383-388.
Carla C.C.R. de Carvalho, M. Manuela R. da Fonseca (2004) Principal
Component Analysis applied to Bacterial Cell Behaviour in the Presence of
Organic Solvents, Biocatalysis and Biotransformation, 22(3), 203-214.
Carla C.C.R. de Carvalho, A. Cruz, M. N. Pons, H.M. Pinheiro, J.M.S.
Cabral, M.M.R. da Fonseca, P. Fernandes, B.S. Ferreira (2004) Mycobacterium sp., Rhodococcus
erythropolis and Pseudomonas putida
behaviour in the presence of organic solvents, Microscopy Research and
Technique, 64(3), 215-222.
Carla C.C.R. de Carvalho, A. Cruz, B. Angelova, P. Fernandes, M.N. Pons,
H.M. Pinheiro, J.M.S. Cabral, M.M.R. da Fonseca (2004) Mycobacterium sp. NRRL B-3805 behaviour in aqueous, organic-aqueous
and organic media studied by fluorescence microscopy, Applied Microbiology
and Biotechnology, 64, 695-701.
Carla C.C.R. de Carvalho, Marie-Nöelle Pons, M. Manuela R. da Fonseca
(2003) Principal Components Analysis as a Tool to Summarise
Biotransformation Data: Influence on Cells of Solvent Type and Phase Ratio,
Biocatalysis and Biotransformation, 21(6), 305-314.
Carla C.C.R. de Carvalho and M. Manuela R. da Fonseca (2003) Towards
the bio-production of trans-carveol and carvone from limonene: induction after
cell growth on limonene and toluene. Tetrahedron: Asymmetry, 14,
3925-3931.
Carla C.C.R. de Carvalho, M. Manuela R. da Fonseca (2003) A Simple
Method to Observe Organic Solvent Drops with a Standard Optical Microscope,
Microscopy Research and Technique, 60 (4), 465-466
Carla C.C.R. de Carvalho, M. Manuela R. da Fonseca (2002) Influence
of Reactor Configuration on the Production of Carvone from Carveol by Whole
Cells of Rhodococcus erythropolis
DCL14, Journal of Molecular Catalysis B: Enzymatic, 19-20C, 377-387
Carla
C.C.R. de Carvalho, M. Manuela R. da Fonseca (2002) Maintenance of Cell
Viability in the Biotransformation of (-)-Carveol with Whole Cells of Rhodococcus
erythropolis, Journal of Molecular Catalysis B: Enzymatic, 19-20C,
389-398
Carla C. C. R. de Carvalho, Frederik van Keulen, M. Manuela R. da Fonseca
(2002) Modelling the bio-kinetic resolution of diastereomers present in
unequal initial amounts, Tetrahedron: Asymmetry, 13 (15), 1637-1643
Carla
C.C.R. de Carvalho, Frederik van Keulen, M. Manuela R. da Fonseca (2000), Production
and recovery of limonene-1,2-diol and simultaneous resolution of a
diastereomeric mixture of limonene-1,2-epoxide with whole cells of Rhodococcus
erythropolis DCL14, Biocatalysis and Biotransformation, 18, 223-235
Carla C.C.R. de Carvalho, Frederik van Keulen, M. Manuela R. da Fonseca
(2000), Biotransformation of limonene-1,2-epoxide to limonene-1,2-diol by Rhodococcus erythropolis cells. An Introductory approach to
selective hydrolysis and product separation, Food Technology and Biotechnology
Journal, 38 (3), 181-185
Carla C. C. R. de Carvalho (2009) “Biotransformation of terpenes by Fungi” in Advances in Fungal Biotechnology (Mahendra Rai, ed.), I.K. International Publishing House Pvt. Ltd, New Delhi, pp. 528, ISBN: 8189866532.
Carla C. C. R. de Carvalho, Bruno S. Ferreira and M. Manuela R. da Fonseca (2007) “Escala laboratorial e escala industrial” in Reactores Biológicos (M. Manuela da Fonseca and José A. Teixeira, eds.), Chapter 7, pp 173-192, Lidel, Portugal.
Carla C. C. R. de Carvalho, Vanessa Fatal, Sebastião S. Alves, M. Manuela R. da Fonseca (2007) Degradation of toluene and xylene by Rhodococcus cells, Journal of Biotechnology, 131(2S), S101.
Carla C. C. R. de Carvalho, Effect of antineoplastic agents on the surface properties of bacterial cells, 2nd World Conference on Magic Bullets - Ehrlich II, Nuremberg, Germany, 3th-5th October 2008.
Carla C.C.R. de Carvalho, Membrane and surface properties of Rhodococcus erythropolis cells in two-phase systems, ESBES 7 - 7th European Symposium on Biochemical Engineering Science, Faro, Portugal, 7th-10th September 2008.
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca, The importance of the bacterial membrane to biofilm formation, IWA Biofilm Technologies Conference, Singapore, 8th-10th January 2008.
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca, Adaptation of Rhodococcus erythropolis cells to degrade high concentrations of toluene, BioMicroWorld, Seville, Spain, 28th November- 1st December 2007.
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca, Rhodococcus erythropolis: a remarkable bacterium for biocatalysis and bioremediation, The 9th International Symposium on Biotechnology, Metal Complexes and Catalysis (BMC-IX), Xilinhot, Inner Mongolia, People’s Republic of China, 26th-29th July 2007.
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca, Influence of reaction conditions on the morphology and physiology of whole cells of Rhodococus erythropolis used as catalyst for the conversion of (-)-carveol to (-)-carvone, 12th International Biotechnology Symposium and Exhibition, Santiago, Chile, 17-22 October 2004.
M.-J. Caramujo and C.C.C.R. Carvalho (2008) Freshwater Biology. 53(1).
Carla C. C. R. de Carvalho, Marco P. C. Marques, Cleaning hydrocarbon containing samples from war vessels, 14th International Biodeterioration and Biodegradation Symposium (IBBS-14), Sant’Alessio Siculo, Messina, Italy, 6th- 11th October 2008.
Carla C. C. R. de Carvalho, Paula S. Ramos-Silva, Sebastião S. Alves, M. Manuela R. da Fonseca, Degradation of linear and aromatic hydrocarbons by Rhodococcus erythropolis cells, 14th International Biodeterioration and Biodegradation Symposium (IBBS-14), Sant’Alessio Siculo, Messina, Italy, 6th- 11th October 2008.
Carla C. C. R. de Carvalho, Marco P. C. Marques, Bioremediation of samples from a Naval Base, ESBES 7 - 7th European Symposium on Biochemical Engineering Science, Faro, Portugal, 7th-10th September 2008.
Carla C. C. R. de Carvalho, Lauro Xavier-Filho, Sheyla Alves Rodrigues, Débora de França Gomes, Priscila Almeida Cruz, Propriedades antimicrobianas do polímero de Abelmoshus esculentus (L.) Moench, Quiabo, 59º Congresso Nacional de Botânica, Natal, Brasil, 2nd-8th August 2008.
Carla C. C. R. de Carvalho and M. Manuela R. da Fonseca, Assessing 3-D biofilm structure with an optical microscope, IWA Biofilm Technologies Conference, Singapore, 8th-10th January 2008.
Carla C. C. R. de Carvalho, Vanessa Fatal, Sebastião S. Alves, M. Manuela R. da Fonseca, Degradation of toluene and xylene by Rhodococcus cells, 13th European Congress on Biotechnology, Barcelona, Spain, 16th-19th September 2007.
Carla
C. C. R. de Carvalho, M. Manuela R. da Fonseca, Influencing cellular membrane
composition to promote attachment/detachment of cells, International Conference
BIOFILMS II - Attachment and Detachment in Pure and Mixed Cultures,
Carla
C.C.R. de Carvalho, M. Manuela R. da Fonseca, Principal Components Analysis as a
Tool to Summarise Biotransformation Data: Influence on Cells of Solvent Type and
Phase Ratio, CAC-2004, Ninth Chemometrics in Analytical Chemistry Conference,
Carla
C.C.R. de Carvalho, A. Cruz, P. Fernandes, M. Manuela R. da Fonseca, J.M.S.
Cabral, H.M. Pinheiro, Mycobacterium
sp. NRRL B-3805 behaviour in aqueous-organic media: viability and activity
profile towards b-sitosterol,
The 5th International Symposium on Biocatalysis and Biotransformation (BIOTRANS
2001), Darmstadt, Germany, September 2nd-7th 2001
“Jovens Investigadores UTL/Deloitte 2008” award, given by Universidade Técnica de Lisboa and Deloitte.
“Best Paper Award” for the oral presentation entitled “The importance of the bacterial membrane to biofilm formation”, IWA Biofilm Technologies Conference, Singapore, January 2008.
Contacts:
Telephone: +(351) 218 419 594
Fax: +(351) 218 419 062
E-mail: Carla Carvalho
Last updated, Thursday, 23 April 2009